A faecal microbiota signature with high specificity for pancreatic cancer. Issue 7 (8th March 2022)

• Record Type: Journal Article
• Authors: Kartal, Ece; Schmidt, Thomas S B; Molina-Montes, Esther; Rodríguez-Perales, Sandra; Wirbel, Jakob; Maistrenko, Oleksandr M; Akanni, Wasiu A; Alashkar Alhamwe, Bilal; Alves, Renato J; Carrato, Alfredo; Erasmus, Hans-Peter; Estudillo, Lidia; Finkelmeier, Fabian; Fullam, Anthony; Glazek, Anna M; Gómez-Rubio, Paulina; Hercog, Rajna; Jung, Ferris; Kandels, Stefanie; Kersting, Stephan; Langheinrich, Melanie; Márquez, Mirari; Molero, Xavier; Orakov, Askarbek; Van Rossum, Thea; Torres-Ruiz, Raul; Telzerow, Anja; Zych, Konrad; Benes, Vladimir; Zeller, Georg; Trebicka, Jonel; Real, Francisco X; Malats, Nuria; Bork, Peer;
• Other Names: author non-byline.; Malats Núria author non-byline.; Real Francisco X author non-byline.; deMaturana Evangelina López author non-byline.; Gómez-Rubio Paulina author non-byline.; Molina-Montes Esther author non-byline.; Alonso Lola author non-byline.; Márquez Mirari author non-byline.; Milne Roger author non-byline.; Alfaro Ana author non-byline.; Lobato Tania author non-byline.; Estudillo Lidia author non-byline.; Lawlor Rita author non-byline.; Scarpa Aldo author non-byline.; Beghelli Stefania author non-byline.; Sharp Linda author non-byline.; Hidalgo Manuel author non-byline.; Rodríguez Jesús author non-byline.; Carrato Alfredo author non-byline.; Caminoa Alejandra author non-byline.; Guillén-Ponce Carmen author non-byline.; Rodríguez-Garrote Mercedes author non-byline.; Longo-Muñoz Federico author non-byline.; Ferreiro Reyes author non-byline.; Pachón Vanessa author non-byline.; Mar Iglesias Lucas Ilzarbe author non-byline.; Álvarez-Urturi Cristina author non-byline.; Bessa Xavier author non-byline.; Bory Felipe author non-byline.; Márquez Lucía author non-byline.; Poves Ignasi author non-byline.; Burdío Fernando author non-byline.; Grande Luis author non-byline.; Gimeno Javier author non-byline.; Molero Xavier author non-byline.; Guarner Luisa author non-byline.; Balcells Joaquin author non-byline.; Salcedo Mayte author non-byline.; Michalski Christoph author non-byline.; Esposito Irene author non-byline.; Kleeff Jörg author non-byline.; Kong Bo author non-byline.; Löhr Matthias author non-byline.; Huang Jiaqui author non-byline.; Verbeke Caroline author non-byline.; Ye Weimin author non-byline.; Yu Jingru author non-byline.; Perea José author non-byline.; Peláez Pablo author non-byline.; Farré Antoni author non-byline.; Mora Josefina author non-byline.; Martín Marta author non-byline.; Artigas Vicenç author non-byline.; Guarner Carlos author non-byline.; JSancho Francesc author non-byline.; yCajal Teresa Ramón author non-byline.; Greenhalf William author non-byline.; O'Rorke Michael author non-byline.; Murray Liam author non-byline.; Cantwell Marie author non-byline.; MBarberá Víctor author non-byline.; Tardón Adonina author non-byline.; Barneo LuSis author non-byline.; Muñoz Enrique Domínguez author non-byline.; Lozano Antonio author non-byline.; Luaces Maria author non-byline.; Muñoz-Bellvís Luís author non-byline.; Sayagués Manzano JM author non-byline.; Gutíerrrez Troncoso ML author non-byline.; deMatos AOrfao author non-byline.; Gress Thomas author non-byline.; Buchholz Malte author non-byline.; Neesse Albrecht author non-byline.; Crnogorac-Jurcevic Tatjana author non-byline.; Kocher Hemant M author non-byline.; Bhattacharya Satyajit author non-byline.; Tabraham Ajit author non-byline.; Ennis Darren author non-byline.; Dowe Thomas author non-byline.; Radon Tomasz author non-byline.; Silverman Debra T author non-byline.; Easton Douglas author non-byline.; author non-byline.; Hans-Peter Jonel Trebicka author non-byline.; FabianFinkelmeier Erasmus author non-byline.; WenyiGuOlaf Robert Schierwagen author non-byline.; Erhard TycFrank author non-byline.; Stefan Uschner author non-byline.; Kersting Stephan author non-byline.; Langheinrich Melanie author non-byline.; Grützmann Robert author non-byline.; FWeber Georg author non-byline.; Pilarsky Christian author non-byline.;
• Is Part Of: Gut. Volume 71:Issue 7(2022)
• Page Start: 1359
• Page End: 1372
• Publication Date: 2022-03-08
• Subjects: pancreatic cancer -- intestinal microbiology -- cancer prevention -- pancreatic tumours -- screening; Gastroenterology -- Periodicals; 616.33
• DOI: 10.1136/gutjnl-2021-324755
• Languages: English
• ISSNs: 0017-5749

Abstract

Abstract : Background: Recent evidence suggests a role for the microbiome in pancreatic ductal adenocarcinoma (PDAC) aetiology and progression. Objective: To explore the faecal and salivary microbiota as potential diagnostic biomarkers. Methods: We applied shotgun metagenomic and 16S rRNA amplicon sequencing to samples from a Spanish case–control study (n=136), including 57 cases, 50 controls, and 29 patients with chronic pancreatitis in the discovery phase, and from a German case–control study (n=76), in the validation phase. Results: Faecal metagenomic classifiers performed much better than saliva-based classifiers and identified patients with PDAC with an accuracy of up to 0.84 area under the receiver operating characteristic curve (AUROC) based on a set of 27 microbial species, with consistent accuracy across early and late disease stages. Performance further improved to up to 0.94 AUROC when we combined our microbiome-based predictions with serum levels of carbohydrate antigen (CA) 19–9, the only current non-invasive, Food and Drug Administration approved, low specificity PDAC diagnostic biomarker. Furthermore, a microbiota-based classification model confined to PDAC-enriched species was highly disease-specific when validated against 25 publicly available metagenomic study populations for various health conditions (n=5792). Both microbiome-based models had a high prediction accuracy on a German validation population (n=76). Several faecal PDAC marker species were detectable in pancreatic tumour and non-tumour tissue using 16S rRNA sequencing and fluorescence in situ hybridisation. Conclusion: Taken together, our results indicate that non-invasive, robust and specific faecal microbiota-based screening for the early detection of PDAC is feasible.