Cold atmospheric plasma inhibits vesicle transport of human osteosarcoma cells visualized by super‐resolution fluorescence microscopy. Issue 6 (11th February 2022)

• Record Type: Journal Article
• Authors: Zhu, Cheng; Chen, Chong; Guo, Jinsong; Wang, Qun; Rao, Junfeng; Li, Hui; Wu, Xiaodong; Zhuang, Jie
• Is Part Of: Plasma processes and polymers. Volume 19:Issue 6(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-11
• Subjects: cold plasma -- human osteosarcoma cells -- plasma treatment -- super resolution fluorescence microscopy -- vesicle transport; Plasma polymerization -- Periodicals; Plasma-enhanced chemical vapor deposition -- Periodicals; Plasma chemistry -- Periodicals
• DOI: 10.1002/ppap.202100210
• Languages: English
• ISSNs: 1612-8850

Abstract

Abstract: Vesicle transport plays a key role in cancer invasion and metastasis. As a promising cancer treatment modality, the impact of cold atmospheric plasma (CAP) on vesicle transport has been barely examined. In this study, super‐resolution structured illumination microscopy (SIM) was employed to track the intracellular vesicles of osteosarcoma cells. Results indicated that CAP exposure could disintegrate vesicle clusters and individual vesicles. After 20 s of CAP treatment, the average velocity of intracellular vesicles decreased from 0.140 to 0.037 μm/s, while the 60 s maximum displacement declined from 7.76 to 3.26 μm. Significant inhibition of vesicle transport between osteosarcoma cells and human bronchial epithelium was observed after CAP treatment. These findings may shed some light on understanding CAP–cancer cell interactions. Abstract : This study investigated the impact of cold atmospheric plasma (CAP) on the vesicle transport of human osteosarcoma cells by means of super‐resolution fluorescence microscopy. CAP could disintegrate vesicle clusters and individual vesicle, significantly decrease the velocity and maximum displacement of intracellular vesicles. Intercellular vesicle transport between osteosarcoma cells and human bronchial epithelium was also inhibited. These findings may shed some light on understanding CAP–cancer cell interactions.