A dual-emission fluorescence-enhanced probe for hydrogen sulfide and its application in biological imaging. (13th May 2022)

• Record Type: Journal Article
• Authors: Li, Minghao; Jiao, Yang; Duan, Chunying
• Is Part Of: New journal of chemistry. Volume 46:Number 21(2022)
• Page Start: 10184
• Page End: 10190
• Publication Date: 2022-05-13
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/d2nj01195f
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A fluorescence-enhanced probe with unique dual-channel emissions was designed for the detection and bioimaging of hydrogen sulfide. Abstract : Hydrogen sulfide (H2 S) is an emerging gasotransmitter in the regulation of numerous physiological and pathological processes. Herein, we reported a dual-emission fluorescence-enhanced probe DCH-S for the detection of biological H2 S. The probe is composed of a coumarin-SBD hybrid and a recognition group of 2, 4-dinitrobenzenesulfonate (DNBS). In response to H2 S, DCH-S rapidly changed into DCH with elimination of the DNBS group and then exhibited a fluorescence enhancement at both 456 nm and 600 nm. The fluorescence intensities reached a plateau within 5 min and the limit of detection was calculated as 47 nM. DCH-S showed high selectivity and anti-interference ability to H2 S over other related analytes. DCH-S was also found to function efficiently under a wide pH range of 5–9 and display low toxicity to living MCF-7 cells. More importantly, DCH-S was successfully applied to detect exogenous/endogenous H2 S in living cells and zebrafish via dual-channel imaging.