Noninvasive examination of the cardiac properties of insect embryos enabled by optical coherence tomography. Issue 6 (14th March 2022)

• Record Type: Journal Article
• Authors: Su, Ya; Fan, Jiangling; Wang, Xiuli; Wang, Xiaoxiao; Li, Jing; Duan, Bingbing; Kang, Le; Wei, Liya; Yao, X. Steve
• Is Part Of: Journal of biophotonics. Volume 15:Issue 6(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-03-14
• Subjects: cardiac function -- dorsal vascular -- embryos -- heart rate -- locust -- nitric oxide synthase -- optical coherence tomography -- RNA interference; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.202100308
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: Understanding the cardiac properties of insect embryos at different development stages is important, however, few works have been conducted probably due to the lack of effective tools. Using locust embryos as an example, here we show, for the first time, that optical coherence tomography (OCT) is capable of obtaining detailed information of embryos' heart activities and irregularities, such as the heart rate, cardiac cycle, diastolic and systolic diameters, hemolymph pumping rate and ejection fraction at different stages of embryonic development and at different temperatures. We develop algorithms and mathematical methods for extracting and analyzing cardiac behavior information of locust embryos. We discover that locust embryos experienced suspended development (quiescence) caused by cold storage have a heart rate 20% more than that of embryos without experiencing quiescence and that the hemolymph pumping rate of the two types of embryos behaves differently as the embryos grow. In addition, using OCT as an accurate cardiac activity examination tool, we show that the heart rates of locust embryos are effectively reduced due to nitric oxide synthase gene silencing by RNA interference, indicating potential application of using locust embryos as a good model organism to study cardiovascular diseases, including the congenital heart disease and arrhythmia. Finally, the capabilities offered by OCT in the studies of locust embryonic development may also prove helpful to promote locust reproduction for nutritions or restrain locust reproduction for pest control. Abstract : We propose and demonstrate the first use of optical coherence tomography (OCT) to noninvasively exam the cardiac functions of insects' embryos as they develop, providing the ability to fast screen the effectiveness on the embryos' heart activities when certain genes are suppression by RNA interference. Applying the OCT capabilities with locusts as the model organism is also beneficial to better understand the human congenital diseases.