Development and single‐particle analysis of hybrid extracellular vesicles fused with liposomes using viral fusogenic proteins. Issue 6 (30th April 2022)

• Record Type: Journal Article
• Authors: Ishikawa, Raga; Yoshida, Shosuke; Sawada, Shin‐ichi; Sasaki, Yoshihiro; Akiyoshi, Kazunari
• Other Names: López‐Lastra Marcelo guestEditor.
• Is Part Of: FEBS open bio. Volume 12:Issue 6(2022)
• Page Start: 1178
• Page End: 1187
• Publication Date: 2022-04-30
• Subjects: baculovirus‐expression system -- extracellular vesicle -- liposome -- membrane fusion -- single‐particle analysis -- viral fusogenic protein; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.13406
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Extracellular vesicles (EVs) have potential biomedical applications, particularly as a means of transport for therapeutic agents. There is a need for rapid and efficient EV‐liposome membrane fusion that maintains the integrity of hybrid EVs. We recently described Sf9 insect cell‐derived EVs on which functional membrane proteins were presented using a baculovirus‐expression system. Here, we developed hybrid EVs by membrane fusion of small liposomes and EVs equipped with baculoviral fusogenic proteins. Single‐particle analysis of EV‐liposome complexes revealed controlled introduction of liposome components into EVs. Our findings and methodology will support further applications of EV engineering in biomedicine. Abstract : We developed hybrid extracellular vesicles by membrane fusion of small liposomes and insect cell‐derived extracellular vesicles equipped with baculoviral fusogenic proteins. Single‐particle analysis of extracellular vesicle‐liposome complexes revealed controlled introduction of liposome components into extracellular vesicles via baculoviral protein‐mediated membrane fusion.