Effect of dietary dihydromyricetin supplementation on lipid metabolism, antioxidant capacity and skeletal muscle fiber type transformation in mice. Issue 3 (1st June 2022)

• Record Type: Journal Article
• Authors: Guo, Zhongyang; Chen, Xiaoling; Huang, Zhiqing; Chen, Daiwen; Yu, Bing; He, Jun; Yan, Hui; Zheng, Ping; Luo, Yuheng; Yu, Jie; Chen, Hong
• Is Part Of: Animal biotechnology. Volume 33:Issue 3(2022)
• Page Start: 555
• Page End: 562
• Publication Date: 2022-06-01
• Subjects: Dihydromyricetin -- lipid metabolism -- antioxidant capacity -- muscle fiber type transformation -- mitochondrial function; Animal biotechnology -- Periodicals; Animals, Domestic -- genetics -- Periodicals; Animals, Genetically Modified -- Periodicals; 660.6
• DOI: 10.1080/10495398.2021.2006204
• Languages: English
• ISSNs: 1049-5398

Abstract

Abstract: This study aimed to investigate the effect of dietary dihydromyricetin (DHM) supplementation on lipid metabolism, antioxidant capacity and muscle fiber type transformation. Twenty-four male Kunming mice were randomly allotted to either control (basal diet) or DHM diets (supplemented with 300 mg/kg DHM). Our data showed that DHM administration decreased the triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) contents, and increased the catalase (CAT), total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) activities in serum. In the liver, DHM decreased the TG and malondialdehyde (MDA) levels and increased the T-SOD and GSH-Px activities. For the tibialis anterior (TA) muscle, DHM increased the total antioxidant capacity (T-AOC) level and T-SOD activities. Western blotting and real-time quantitative PCR analysis showed that DHM increased the protein and mRNA levels of MyHC I and MyHC IIa and decreased the protein and mRNA levels of MyHC IIb in TA muscle, which may be achieved by activating the AMP-activated protein kinase (AMPK) signal. The mRNA levels of several regulatory factors related to mitochondrial function were up-regulated by DHM. In conclusion, dietary 300 mg/kg DHM supplementation improved lipid metabolism and antioxidant capacity and promoted the transformation of muscle fiber type from glycolysis to oxidation in mice.