Nano-titanium nitride causes developmental toxicity in zebrafish through oxidative stress. (4th July 2022)

• Record Type: Journal Article
• Authors: Wang, Jiazhen; Liu, Kechun; Mo, Cailian; Minchenko, Oleksandr H.; Zhang, Yun; Chen, Jung-Ren; Hsiao, Chung-Der; Zhu, Qing; He, Qiuxia
• Is Part Of: Drug and chemical toxicology. Volume 45:Number 4(2022)
• Page Start: 1660
• Page End: 1669
• Publication Date: 2022-07-04
• Subjects: Nano-titanium nitride -- Danio rerio -- development -- oxidative stress -- Nrf2; Toxicology -- Periodicals; Drugs -- Toxicology -- Periodicals; Toxicology, Experimental -- Periodicals; 615.9005
• DOI: 10.1080/01480545.2020.1853765
• Languages: English
• ISSNs: 0148-0545

Abstract

Abstract: Nano-titanium nitride (Nano-TiN) has strong resistance to wear and corrosion, good biocompatibility, and an attractive metallic luster. Nano-TiN is widely used in medical devices, such as orthopedic implants, syringe needles, coronary stents, and long-term dental implants, and also in imitation gold jewelry. Despite its widespread use, there are few reports describing safety evaluations of Nano-TiN. Here, we exposed healthy zebrafish embryos to different concentrations of Nano-TiN solution for five days, starting at about four hours post fertilization, and found that Nano-TiN caused dose- and time-dependent developmental toxicity. With increasing Nano-TiN concentration and length of exposure, mortality, and deformities gradually increased; body length shortened and hatching rate and motility were significantly reduced. We also found that exposure to Nano-TiN affected development of the heart, liver, nerves, and other organs, and led to elevated levels of reactive oxygen species and reduced antioxidant capacity. Exposure to Nano-TiN resulted in downregulation of expression of antioxidant genes, such as nrf2, gclc, gclm, ho-1, and nqo1 . Our results showed that exposure to Nano-TiN caused developmental and organ toxicity in zebrafish embryos and that the toxic effects may be mediated through oxidative stress.