Increased acrolein–DNA adducts in buccal brushings of e-cigarette users. (3rd March 2022)

• Record Type: Journal Article
• Authors: Cheng, Guang; Guo, Jiehong; Carmella, Steven G; Lindgren, Bruce; Ikuemonisan, Joshua; Niesen, Brittany; Jensen, Joni; Hatsukami, Dorothy K; Balbo, Silvia; Hecht, Stephen S
• Is Part Of: Carcinogenesis. Volume 43:Number 5(2022)
• Page Start: 437
• Page End: 444
• Publication Date: 2022-03-03
• Subjects: Carcinogenesis -- Periodicals; Cancer -- Genetic aspects -- Periodicals; Cancer -- Prevention -- Periodicals; Cancer -- Periodicals; 616.994071
• DOI: 10.1093/carcin/bgac026
• Languages: English
• ISSNs: 0143-3334

Abstract

Abstract: DNA adducts are central in the mechanism of carcinogenesis by genotoxic agents. We compared levels of a DNA adduct of acrolein, a genotoxic carcinogen found in e-cigarette vapor, in oral cell DNA of e-cigarette users and non-users of any tobacco or nicotine product. e-Cigarette users and non-users visited our clinic once monthly for 6 months, and oral brushings and urine samples were collected. For this study, we analyzed oral cell DNA adducts from three monthly visits in e-cigarette users and non-users as confirmed by urinary cyanoethyl mercapturic acid and total nicotine equivalents. DNA was isolated from the oral brushings and analyzed by a validated liquid chromatography-nanoelectrospray ionization-high resolution tandem mass spectrometry method for the acrolein DNA adduct 8 R / S -3-(2'-deoxyribos-1'-yl)-5, 6, 7, 8-tetrahydro-8-hydroxypyrimido[1, 2- a ]purine-10-(3 H )-one (γ-OH-Acr-dGuo). The median value of this DNA adduct in the e-cigarette users was 179 fmol/µmol dGuo (range 5.0 - 793 fmol/µmol dGuo) while that for non-users was 21.0 fmol/µmol dGuo (range 5.0 - 539 fmol/µmol dGuo), P = 0.001. These results demonstrate for the first time that e-cigarette users have elevated levels of a carcinogen–DNA adduct in their oral cells. Abstract : DNA adducts of acrolein were significantly greater in oral cells of 20 e-cigarette users sampled monthly for 3 months than in 20 non-users. This is the first identification of a carcinogen–DNA adduct in any tissue of an e-cigarette user. Graphical Abstract: