A Sensitive Liquid Chromatography-Mass Spectrometry Method for Determination of Toosendanin in Rat Plasma and its Application to Pharmacokinetic Study. (21st December 2021)

• Record Type: Journal Article
• Authors: Shen, Chuangpeng; Pan, Zhisen; Wu, Xiaojie; Zhong, Chong; Li, Qiao; Si, Yuqi; Liu, Changhui; Tu, Haitao; Deng, Zhijun; Zhu, Zhangzhi; Guo, Jiewen; Xin, Xiaoyi; Liu, Min
• Is Part Of: Journal of chromatographic science. Volume 60:Number 5(2022)
• Page Start: 478
• Page End: 485
• Publication Date: 2021-12-21
• Subjects: Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1093/chromsci/bmab135
• Languages: English
• ISSNs: 0021-9665

Abstract

Abstract: A simple, rapid and sensitive analytical method was developed for the determination of toosendanin in rat plasma using liquid chromatography tandem mass spectrometry (LC–MS/MS). Andrographolide was selected as the internal standard, and the plasma samples were extracted by liquid–liquid extraction with diethyl ether. Chromatographic separation was performed on a Dikma Spursil C18, 3.5 μm (150 × 2.1 mm i.d) analytical column with 85% methanol:water (v/v) containing 0.025% formic acid (pH = 3.9) as mobile phase. The flow rate was 0.25 mL/min, and the total run time was 3 min. Detection was performed with a triple-quadrupole tandem mass spectrometer using negative ion mode electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode. The MS/MS ion transitions monitored were m/z 573.1 → 531.1 and 349.0 → 287.0 for toosendanin and andrographolide, respectively. Good linearity was observed over the concentration range of 3.125–500 ng/mL in 100 μL of rat plasma with a correlation coefficient ˃0.9997. Intra- and inter-assay variabilities were ˂8.5% in plasma. The recovery and the matrix effect were in the range 71.8–73.5% and 96.4–103.8%, respectively. The analyte was stable under various conditions (at room temperature, during freeze–thaw settings, in the autosampler, and under deep-freeze conditions). The method was successfully applied to a pharmacokinetic study of toosendanin after its oral administration in rats at a dose of 10 mg/kg.