CRISPR-based metabolic engineering in non-model microorganisms. (June 2022)

• Record Type: Journal Article
• Authors: Lu, Liangyu; Shen, Xiaolin; Sun, Xinxiao; Yan, Yajun; Wang, Jia; Yuan, Qipeng
• Is Part Of: Current opinion in biotechnology. Volume 75(2022)
• Publication Date: 2022-06
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2022.102698
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: Non-model microorganisms serve as promising industrial chassis for bioproduction. CRISPR-based genetic modifications enable desired production phenotypes. CRISPR-mediated genome-wide perturbations allows for metabolic engineering of non-model microorganisms. Precise pathway engineering requires CRISPR-mediated editing tools with improved editing efficiency. Abstract : Non-model microorganisms possess unique and versatile metabolic characteristics, offering great opportunities as cell factories for biosynthesis of target products. However, lack of efficient genetic tools for pathway engineering represents a big challenge to unlock the full production potential of these microbes. Over the past years, CRISPR systems have been extensively developed and applied to domesticate non-model microorganisms. In this paper, we summarize the current significant advances in designing and constructing CRISPR-mediated genetic modification systems in non-model microorganisms, such as bacteria, fungi and cyanobacteria. We particularly put emphasis on reviewing some successful implementations in metabolic pathway engineering via CRISPR-based genome editing tools. Moreover, the current barriers and future perspectives on improving the editing efficiency of CRISPR systems in non-model microorganisms are also discussed.