Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC). (August 2022)

• Record Type: Journal Article
• Authors: Kaushal, Jyoti; Arya, Shailendra Kumar; Khatri, Madhu; Singh, Gursharan; Izyan Wan Azelee, Nur; Rajagopal, Rajinikanth; Woong Chang, Soon; Ravindran, Balasubramani; Kumar Awasthi, Mukesh
• Is Part Of: Bioresource technology. Volume 357(2022)
• Publication Date: 2022-08
• Subjects: Xylooligosaccharides (XOS) -- Xylanase -- Hemicellulosic -- Metal organic framework -- Immobilization -- Xylotetrose -- Xylopentose; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127374
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Xylanase (Xy) extracted from Bacillus pumilus bacterial strain was partially purified. Production of Cu-BTC MOF was done at room temperature. Xy was immobilized onto Cu-BTC MOF to form blue crystals of Xy-Cu-BTC. Xylan was extracted from waste walnut shell. Bioconversion of xylan to xylooligosaccharides by free and immobilized enzyme system. Abstract: This study uses a cost effective and efficient method for production of higher DP (degree of polymerization) Xylooligosaccharides (XOS) from xylan extracted from the waste walnut shells. Copper based metal organic framework (Cu-BTC MOF) was prepared for immobilization of free xylanase (Xy) enzyme by green synthesis method. Both free and immobilized xylanase (Xy-Cu-BTC) were able to cause the bioconversion of xylan (87.4% yield) into XOS. Predominant production of xylotetrose (X4) and xylopentose (X5) was observed for both the methods. Percentage XOS conversion for free enzyme (Xy) was found to be 4.1% X4 and 60.57% X5 whereas these values increased in case of immobilized system where 11.8% X4 and 64.2% X5 were produced. Xylose production was minute in case of immobilized xylanase 0.88% which makes it a better method for XOS production free from xylose interference. Xy-Cu-BTC MOF can hence be used as an attractive alternative for pure XOS production.