Dissipative Control over the Toehold‐Mediated DNA Strand Displacement Reaction. (5th April 2022)

• Record Type: Journal Article
• Authors: Del Grosso, Erica; Irmisch, Patrick; Gentile, Serena; Prins, Leonard J.; Seidel, Ralf; Ricci, Francesco
• Is Part Of: Angewandte Chemie. Volume 134:Number 23(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-04-05
• Subjects: DNA Nanotechnology -- Dissipative Self-Assembly -- Strand Displacement Reaction -- Temporal Control; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202201929
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Here we show a general approach to achieve dissipative control over toehold‐mediated strand‐displacement, the most widely employed reaction in the field of DNA nanotechnology. The approach relies on rationally re‐engineering the classic strand displacement reaction such that the high‐energy invader strand (fuel) is converted into a low‐energy waste product through an energy‐dissipating reaction allowing the spontaneous return to the original state over time. We show that such dissipative control over the toehold‐mediated strand displacement process is reversible (up to 10 cycles), highly controllable and enables unique temporal activation of DNA systems. We show here two possible applications of this strategy: the transient labelling of DNA structures and the additional temporal control of cascade reactions. Abstract : How to rationally re‐engineer the classic strand displacement reaction is reported such that the high‐energy fuel strand is converted into a low‐energy waste product through an energy‐dissipating reaction, allowing the spontaneous return to the original state. Two possible applications are also demonstrated: the transient labelling of DNA structures and the additional temporal control of cascade reactions.