A sensitive electrochemical DNA sensor based on reduced graphene oxide modified electrode. Issue 5 (30th May 2022)

• Record Type: Journal Article
• Authors: Xu, Binxiang; Hu, Yuping; Shu, Qingxia; Wang, Mei; Chen, Zhiyang; Wei, Wei; Wen, Jinmei; Li, Rui; Liao, Fusheng; Cheng, Lin; Fan, Hao
• Is Part Of: Journal of the Chinese Chemical Society. Volume 69:Issue 5(2022)
• Page Start: 822
• Page End: 830
• Publication Date: 2022-05-30
• Subjects: DNA walker -- electrochemical detection -- reduced graphene oxide -- signal amplification -- tetraferrocene; Chemistry -- Periodicals; Electronic journals; 540.5
• DOI: 10.1002/jccs.202200050
• Languages: English
• ISSNs: 0009-4536

Abstract

Abstract: In this work, an electrochemical sensing platform, analogous to a DNA walker, was explored with exonuclease III (EXO III) as a driving force, tetraferrocene as a signal marker, and reduced graphene oxide (RGO) electrode as two‐dimensional (2D) orbit to realize ultrasensitive detection of target DNA. We designed a single hairpin DNA probe with 3′‐end modified tetraferrocene, immobilized on the electrode surface by π‐π stacking between the 5′ terminus and reduced graphene oxide. In addition, EXO III digested the double‐stranded DNA with a blunt 3′ terminus formed by the target DNA and the probe DNA, thus degrading the 3′ end of probe DNA, and resulting in the away movement of tetraferrocene from the electrode surface, decreasing the electrochemical signal. The proposed electrochemical sensor was very simple and stable with acceptable reproducibility, excellent DNA detection performance, and a detection limit as low as 0.3 pM. Abstract : Schematic illustration of a novel electrochemical sensing platform for the detection of DNA.