A hederagenin-type triterpene saponin, sumbulianoside a from Cephalaria sumbuliana and its potent immunomodulatory activity against seasonal flu virus H3N2. Issue 10 (23rd May 2022)

• Record Type: Journal Article
• Authors: Abaci, Huseyin; Akagac, Gulsah; Nalbantsoy, Ayse; Sarikahya, Nazli Boke
• Is Part Of: Natural product research. Volume 36:Issue 10(2022)
• Page Start: 2495
• Page End: 2503
• Publication Date: 2022-05-23
• Subjects: Cephalaria sumbuliana -- Caprifoliaceae -- sumbulianoside A -- saponin -- cytotoxic activity -- immunomodulatory activity -- H3N2; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2021.1910691
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: A new hederagenin-type triterpene saponin; hederagenin 28-O- β -D-galactopyranosyl-(1→6)- β -D-glucopyranosyl ester named sumbulianoside A (1 ), together with twelve known saponins were isolated from the n -butanol extract of Cephalaria sumbuliana (Caprifoliaceae) from which, one known saponin, dipsacus saponin A (2 ) was isolated, for the first time from Cephalaria species. The structures of the isolated compounds were elucidated by 1 D and 2 D NMR and HRESIMS analyses. Cytotoxic activities were investigated on A549, Hela, PANC1, SHSY5Y cells and non-cancerous cell HEK293 by MTT method and immunomodulatory activities were evaluated against activated H3N2 seasonal virus in whole blood by measuring IL-4, IFN- γ, IL-1 β cytokine level with ELISA. According to the cytotoxicity results, compounds 1 and 2 did not possess significant cytotoxicity, while only compound 2 induced significant IL-4 production (** p <.001) against H3N2 showing a possible Th2 response and antibody production. All compounds had no effect on cytokine release ( p > 0.5). Graphical Abstract: UF0001