Flow Injection Analysis (FIA) with Thermal Lens Spectrometry (TLS) for the Rapid Determination of Cefoperazone in Urine and Water. (2nd June 2022)

• Record Type: Journal Article
• Authors: Soto, César; Saavedra, Renato; Contreras, David; Poza, Cristián; Orellana, Sandra; Olivares-Rentería, Georgina A.
• Is Part Of: Analytical letters. Volume 55:Number 12(2022)
• Page Start: 1932
• Page End: 1944
• Publication Date: 2022-06-02
• Subjects: Antibiotics -- cefoperazone -- flow injection analysis (FIA) -- solid-phase extraction (SPE) -- thermal lens spectrometry (TLS); Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2022.2036181
• Languages: English
• ISSNs: 0003-2719

Abstract

Abstract: An analytical method combining flow-injection analysis (FIA) with thermal-lens spectrometry (TLS) has been developed for high-throughput and sensitive analysis of cefoperazone (CPZ) in tap water and urine samples. The method is based on the indirect determination of CPZ through its alkaline oxidation with KMnO4 . The performance of FIA-TL was validated for the determination of CPZ in the samples. The experimental parameters affecting the reaction were studied and optimized by a multivariate method. The calibration curves for the determination of CPZ were obtained considering the optimum values of the parameters. The detection limits were 2.70·10 −7 mol L −1, 4.88·10 −7 mol L −1, and 1.89·10 −7 mol L −1, in standard solution, tap water, and urine, respectively. This work reports a new solid-phase extraction (SPE) procedure for the analysis of urine. The accuracy of the analytical method was assessed by spike and recovery experiments. The precision was evaluated with inter-day and intra-day trials. The FIA-TLS method shows advantages over the batch conventional operation, such as high sensitivity, simple processing, low volume consumption, and high sample throughput (60 samples hr −1 ). The optimized FIA-TLS method may be applied for fast and sensitive quantitation of CPZ in real samples in flowing media.