Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics. (31st December 2022)

• Record Type: Journal Article
• Authors: Ma, Shan-Peng; Ma, Wei-Ping; Yin, Shi-Ning; Chen, Xiang-Yue; Ma, Xiao-Qing; Wei, Bao-Hong; Lu, Jing-Guang; Liu, Hong-Bing
• Is Part Of: Pharmaceutical biology. Volume 60:Number 1(2022)
• Page Start: 1055
• Page End: 1062
• Publication Date: 2022-12-31
• Subjects: Traditional Chinese medicine -- hyperhidrosis -- pharmacodynamics -- metabolic mechanism; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.1080/13880209.2022.2074465
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context: Mulisan decoction (MLS) is a classic formula of traditional Chinese medicine for treating hyperhidrosis. The mechanism remains unclear. Objective: To investigate the antiperspirant effect and underlying mechanisms of MLS. Materials and methods: Fifty rats were divided into control, model, and three doses of MLS intervention groups ( n = 10). Rats except for control group were induced diseases features of the applicable scope of MLS via i.p. reserpine (0.5 mg/kg/d) for 10 days. From day 11, MLS groups were administrated orally MLS at 0.6, 3, and 15 g/kg once a day for 14 days, respectively. After the last administration, sweating was induced in all rats via s.c. pilocarpine (25 mg/kg), the right hind foot of rats was stained, and sweat point numbers were observed. Rat serum was collected to detect IL-2, IL-6, IFN-γ, and TNF-α. Rat plasma was collected for endogenous metabolite analysis via UPLC-QE-Focus-MS. Results: Rats treated with MLS presented a significant decrease in sweat point numbers (13.5%), increase in body weight (13.2%), and promotion in the balance of Th1/Th2 cytokine ratio via increasing IL-2 (38.3%), IFN-γ (20.1%), and TNF-α (22.0%) and decreasing IL-6 (24.7%) compared with the model group ( p < 0.05). Plasma metabolomics disclosed 15 potential biomarkers related to model rats, of which two could be significantly reversed by MLS ( p < 0.05). The involved pathways were pantothenate and CoA biosynthesis, and porphyrin metabolism. Conclusions: MLS demonstrated a good antiperspirant effect and metabolism improvement. These findings inspire more clinical study validation on immune improvement and antiperspirant effect.