H2O2-activated independently bidirectional regulation for a sensitive and accurate electrochemiluminescence ratiometric analysis. Issue 11 (10th May 2022) - Liu, Xiang-Mei
Abstract : A novel ECL ratiometric sensor was developed based on H2 O2 activated independently bidirectional regulation strategy. Abstract : Potential-resolved electrochemiluminescence (ECL) ratiometric analysis has become a research hotspot in bioassays by virtue of its good accuracy, versatility and specificity. Current ECL ratiometry mainly focuses on the competition for the co-reactant or quantitative analysis using a variable signal and a changeless signal; the disorganized change or small difference between the two signals may affect the accuracy and sensitivity of detection. In this study, we have developed a novel ECL ratiometric sensor based on the bidirectional regulation of two independent co-reaction systems by H2 O2 . H2 O2 as a bidirectional moderator permits the ECL signals of the cathode and anode to independently change in opposite trends, which greatly enhances the organization and difference between the two signals. The ratio of the two signals is used to realize the quantitative analysis of myoglobin (MyO) with a good linear relationship between log(ECLcathode /ECLanode ) and log C MyO in the range of 1.0 × 10 −13 to 1.0 × 10 −7 g mL −1 . The detection limit is 4.0 × 10 −14 g mL −1 . Furthermore, it showed excellent performance in the determination of MyO in human serum samples. The proposed biosensor provides some developments for the sensitive and accurate detection of disease markers.