A magnet-actuated microfluidic array chip for high-throughput pretreatment and amplification and detection of multiple pathogens. Issue 11 (5th May 2022)

• Record Type: Journal Article
• Authors: Zhou, Mengfan; Su, Huiying; Wang, Bangfeng; Wan, Chao; Du, Wei; Chen, Peng; Feng, Xiaojun; Liu, Bi-Feng
• Is Part Of: Analyst. Volume 147:Issue 11(2022)
• Page Start: 2433
• Page End: 2441
• Publication Date: 2022-05-05
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d2an00430e
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : The outbreak of global infectious diseases has posed a significant threat to public health, requiring the rapid and accurate diagnosis of pathogens promptly for society to implement immediate control measures to prevent widespread pandemics. Abstract : The outbreak of global infectious diseases has posed a significant threat to public health, requiring the rapid and accurate diagnosis of pathogens promptly for the society to implement immediate control measures to prevent widespread pandemics. In this work, a magnet-actuated microfluidic array chip (MMAC) is developed with integrated sample processing and nucleic acid amplification for the rapid detection of multiple pathogens by loop-mediated isothermal amplification. In comparison to previous works, where fluid control was dependent on external equipment or finger-based manual pressing, the fluid control of the MMAC is realized by magnetically actuating a ferric oxide (Fe3 O4 ) doped polydimethylsiloxane (PDMS) layer that separates the sample from the LAMP reagent in a high-throughput manner, which not only reduces the complexity of fluid control but also enhances the repeatability of detection by eliminating variations in operation by different users. Examination with a testing sample containing Salmonella typhimurium and Escherichia coli showed high specificity for pathogen detection without cross-contamination. The lowest detection concentration was 5.2 copies per μL for Salmonella typhimurium with a detection time of 60 min. The proposed method demonstrated the simultaneous detection of multiple pathogens, which is potentially helpful in applications of immediate diagnosis.