Aptazyme-induced cascade amplification integrated with a volumetric bar-chart chip for highly sensitive detection of aflatoxin B1 and adenosine triphosphate. Issue 11 (10th May 2022)

• Record Type: Journal Article
• Authors: Han, Yuanyue; Liu, Xinli; Zhao, Qiao; Gao, Yanfeng; Zhou, Dongtao; Long, Wenxiu; Wang, Yuzhen; Song, Yujun
• Is Part Of: Analyst. Volume 147:Issue 11(2022)
• Page Start: 2500
• Page End: 2507
• Publication Date: 2022-05-10
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d2an00650b
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A triple-channel volumetric bar-chart chip based on aptazyme-induced cascade signal amplification empowers visual readout of aflatoxin B1 and adenosine triphosphate concentration. Abstract : A simple aptazyme-induced cascade signal amplification (denoted as ACSA) was integrated with a triple-channel volumetric bar-chart chip (TV-Chip) to visually quantitate aflatoxin B1 (AFB1) and adenosine triphosphate (ATP). Bifunctional aptazymes consisting of aptamers and G-quadruplex-forming sequences were modified on magnetic silica nanoparticles (MSNPs) to construct sensing probes. The recognition of aptamers and targets leads to the formation of hemin/G-quadruplex (hGQ) DNAzymes, which can mimic the horseradish peroxidase (HRP)-accelerated signal enhancement reaction, enabling the polymerization of dopamine and subsequent deposition of polydopamine (PDA) on the MSNP probes, thereby providing abundant anchor sites to covalently immobilize numerous 4-mercaptophenylboric acid (4-MPBA)-modified platinum nanoparticles (PtNPs) (MPt). As a result, this strategy possesses high sensitivity by introducing a large number of PtNPs into the TV-Chip. The detection limits of AFB1 and ATP with 0.075 pM and 0.818 pM, respectively, were easily achieved without any additional instruments. In addition, the detection results of AFB1-spiked food samples were in good agreement with the commercial AFB1 ELISA kit, which verified the accuracy and reliability of this method. The ACSA-based TV-Chip would show great promise for on-site and real-time visual quantitation of trace targets.