Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles. (23rd September 2021)

• Record Type: Journal Article
• Authors: Ong, Sean Wei Xiang; Hon, Pei Yun; Wee, Sharon Syn Hui; Chia, Jonathan Wei Zhong; Mendis, Shehara; Izharuddin, Ezlyn; Lin, Ray Junhao; Chia, Po Ying; Sim, Rees Chin Swee; Chen, Mark I-Cheng; Chow, Angela; Yoong, Joanne; Lye, David Chien; Teng, Christine B; Tambyah, Paul Anantharajah; Banerjee, Ritu; Patel, Robin; De, Partha Pratim; Vasoo, Shawn
• Is Part Of: Clinical infectious diseases. Volume 74:Number 10(2022)
• Page Start: 1850
• Page End: 1854
• Publication Date: 2021-09-23
• Subjects: antimicrobial resistance -- gram-negative bacteria -- diagnostics -- rapid test kits -- ESBL -- carbapenemase; Communicable diseases -- Periodicals; 616.905
• DOI: 10.1093/cid/ciab848
• Languages: English
• ISSNs: 1058-4838

Abstract

Abstract: We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.