Novosphingobium percolationis sp. nov. and Novosphingobium huizhouense sp. nov., isolated from landfill leachate of a domestic waste treatment plant. Issue 5 (27th May 2022)

• Record Type: Journal Article
• Authors: He, Xiaoling; Lu, Huibin; Hu, Wenzhe; Deng, Tongchu; Gong, Xiaofan; Yang, Xunan; Song, Da; He, Mei; Xu, Meiying
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 5(2022)
• Publication Date: 2022-05-27
• Subjects: Novosphingobium -- landfill leachate -- new species -- identification; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005394
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Two strains designated as c1 T and c7 T, were isolated from the landfill leachate of a domestic waste treatment plant in Huizhou City, Guangdong Province, PR China. The cells of both strains were aerobic, rod-shaped, non-motile and formed yellow colonies on Reasoner's 2A agar plates. Strain c1 T grew at 10–42 °C (optimum, 30 °C), pH 4.5–10.5 (optimum, pH 7.0) and 0–2.0 % (w/v) NaCl (optimum, 0–0.5 %). Strain c7 T grew at 10–42 °C (optimum, 30 °C), pH 4.5–10.5 (optimum, pH 6.0) and 0–2.0 % (w/v) NaCl (optimum, 0–0.5 %). Phylogenetic analyses revealed that strains c1 T and c7 T belong to the genus Novosphingobium . The 16S rRNA gene sequence similarities of strains c1 T and c7 T to the type strains of Novosphingobium species were 94.5–98.2 % and 94.3–99.1 %, respectively. The calculated pairwise average nucleotide identity values among strains c1 T, c7 T and the reference strains were in the range of 75.2–85.9 % and the calculated pairwise average amino acid identity values among strains c1 T, c7 T and reference strains were in the range of 72.0–88.3 %. Their major respiratory quinone was Q-10, and the major cellular fatty acids were C18 : 1 ω 7 c, C18 : 0, C16 : 1 ω 7 c, C16 : 0 and C14 : 0 2OH. The major polar lipids of strains c1 T and c7 T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, sphingoglycolipid, unidentified lipids and unidentified phospholipid. Based on phenotypic, chemotaxonomic, phylogenetic and genomic results from this study, strains c1 T and c7 T should represent two independent novel species of Novosphingobium, for which the names Novosphingobium percolationis sp. nov. (type strain c1 T =GDMCC 1.2555 T =KCTC 82826 T ) and Novosphingobium huizhouense sp. nov. (type strain c7 T =GDMCC 1.2556 T =KCTC 82827 T ) are proposed. The gene function annotation results of strains c1 T and c7 T suggest that they could play an important role in the degradation of organic pollutants.