Biodegradation of 4-nitroaniline by novel isolate Bacillus sp. strain AVPP64 in the presence of pesticides. (1st August 2022)

• Record Type: Journal Article
• Authors: Silambarasan, Sivagnanam; Cornejo, Pablo; Vangnai, Alisa S.
• Is Part Of: Environmental pollution. Volume 306(2022)
• Publication Date: 2022-08-01
• Subjects: Bacillus sp. strain AVPP64 -- 4-nitroaniline -- Biodegradation -- Pesticides tolerance -- Nitrogen fixation -- Phosphate solubilization; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.119453
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: In this study, Bacillus sp. strain AVPP64 was isolated from diuron-contaminated soil. It showed 4-nitroaniline (4-NA) degradation, pesticide tolerance, and self-nutrient integration via nitrogen (N)-fixation and phosphate (P)-solubilization. The rate constant ( k ) and half-life period ( t 1/2 ) of 4-NA degradation in the aqueous medium inoculated with strain AVPP64 were observed to be 0.445 d −1 and 1.55 d, respectively. Nevertheless, in the presence of chlorpyrifos, profenofos, atrazine and diuron pesticides, strain AVPP64 degraded 4-NA with t 1/2 values of 2.55 d, 2.26 d, 2.31 d and 3.54 d, respectively. The strain AVPP64 fixed 140 μg mL −1 of N and solubilized 103 μg mL −1 of P during the presence of 4-NA. In addition, strain AVPP64 produced significant amounts of plant growth-promoting metabolites like indole 3-acetic acid, siderophores, exo-polysaccharides and ammonia. In the presence of 4-NA and various pesticides, strain AVPP64 greatly increased the growth and biomass of Vigna radiata and Crotalaria juncea plants. These results revealed that Bacillus sp. strain AVPP64 can be used as an inoculum for bioremediation of 4-NA contaminated soil and sustainable crop production even when pesticides are present. Graphical abstract: Image 1 Highlights: Bacillus sp. strain AVPP64 is a 4-nitroaniline (4-NA) degrading bacterium. Strain AVPP64 showed the ability to tolerate a number of pesticides. Effect of pesticides on 4-NA biodegradation was evaluated. 4-NA biodegradation pathway was proposed. Strain AVPP64 showed plant growth-promoting traits under 4-NA and pesticide stress.