Overview of Peptide and Protein Analysis by Mass Spectrometry. Issue 1 (1st October 2014)

• Record Type: Journal Article
• Authors: Zhang, Guoan; Annan, Roland S.; Carr, Steven A.; Neubert, Thomas A.
• Editors: Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin
• Is Part Of: Current protocols in molecular biology. Volume 108:Issue 1(2014)
• Page Start: 10.21.1
• Page End: 10.21.30
• Publication Date: 2014-10-01
• Subjects: proteomics -- mass spectrometry -- review; Molecular biology -- Technique -- Periodicals; Molecular biology -- Laboratory manuals; Molecular Biology -- methods; Biologie moléculaire -- Technique; Biologie moléculaire -- Manuels de laboratoire; Molecular biology; Molecular biology -- Technique; Laboratory Manual; Electronic reference sources; Laboratory manuals; 572.8028
• DOI: 10.1002/0471142727.mb1021s108
• Languages: English
• ISSNs: 1934-3639

Abstract

Abstract: Mass spectrometry is an indispensable tool for peptide and protein analysis owing to its speed, sensitivity, and versatility. It can be used to determine amino acid sequences of peptides, and to characterize a wide variety of post‐translational modifications such as phosphorylation and glycosylation. Mass spectrometry can also be used to determine absolute and relative protein quantities, and can identify and quantify thousands of proteins from complex samples, which makes it an extremely powerful tool for systems biology studies. The main goals of this unit are to familiarize peptide and protein chemists and biologists with the types of mass spectrometers that are appropriate for the majority of their analytical needs, to describe the kinds of experiments that can be performed with these instruments on a routine basis, and to discuss the kinds of information that these experiments provide. Curr. Protoc. Mol. Biol . 108:10.21.1‐10.21.30. © 2014 by John Wiley & Sons, Inc.