Overview of Target Enrichment Strategies. Issue 1 (1st October 2015)

• Record Type: Journal Article
• Authors: Kozarewa, Iwanka; Armisen, Javier; Gardner, Andrew F.; Slatko, Barton E.; Hendrickson, C.L.
• Editors: Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin
• Is Part Of: Current protocols in molecular biology. Volume 112:Issue 1(2015)
• Page Start: 7.21.1
• Page End: 7.21.23
• Publication Date: 2015-10-01
• Subjects: target enrichment -- DNA capture -- hybridization based enrichment -- PCR amplification; Molecular biology -- Technique -- Periodicals; Molecular biology -- Laboratory manuals; Molecular Biology -- methods; Biologie moléculaire -- Technique; Biologie moléculaire -- Manuels de laboratoire; Molecular biology; Molecular biology -- Technique; Laboratory Manual; Electronic reference sources; Laboratory manuals; 572.8028
• DOI: 10.1002/0471142727.mb0721s112
• Languages: English
• ISSNs: 1934-3639

Abstract

Abstract: Target enrichment is commonly used in next generation sequencing (NGS) workflows to eliminate genomic DNA regions that are not of interest for a particular experiment. By only targeting specific regions such as exons, one can obtain greater depth of DNA sequencing coverage for regions of interest or increase the sampling numbers of individuals, thereby saving both time and cost. This overview of target enrichment strategies provides a high‐level review of distinct approaches to capture specific sequences: (a) hybridization‐based strategies, (b) transposon‐mediated fragmentation (tagmentation), (c) molecular inversion probes (MIPs), and (d) singleplex and multiplex polymerase chain reaction (PCR) target enrichment. Strategies for assay design and performance criteria are also discussed. Other platforms currently in development are also briefly described. © 2015 by John Wiley & Sons, Inc.