Production of Monoclonal Antibodies. (16th February 2018)

• Record Type: Journal Article
• Authors: Yokoyama, Wayne M.; Christensen, Michelle; Santos, Gary Dos; Miller, Diane; Ho, Jason; Wu, Tao; Dziegelewski, Michael; Neethling, Francisca A.
• Editors: Coligan, John E.; Bierer, Barbara E.; Margulies, David H.; Shevach, Ethan M.; Strober, Warren
• Is Part Of: Current protocols in immunology. Volume 102(2013)
• Page Start: 2.5.1
• Page End: 2.5.29
• Publication Date: 2018-02-16
• Subjects: monoclonal antibodies -- B cell hybridomas -- limiting dilution -- semi‐solid methylcellulose‐based medium; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/0471142735.im0205s102
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract: This unit describes the production of monoclonal antibodies beginning with immunization, cell fusion, and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one‐step selection and cloning of hybridomas utilizing a semi‐solid methylcellulose‐based medium (ClonaCell‐HY from StemCell Technologies). Curr. Protoc. Immunol . 102:2.5.1‐2.5.29. © 2013 by John Wiley & Sons, Inc.