Duchesnea indica Extract Ameliorates LPS-Induced Septic Shock in Mice. (14th May 2022)

• Record Type: Journal Article
• Authors: Lee, Yuan Yee; Yuk, Heung Joo; Saba, Evelyn; Kim, Sung Dae; Kim, Dong-Seon; Kopalli, Spandana Rajendra; Oh, Jae-Wook; Rhee, Man Hee
• Other Names: Xie Danli Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2022(2022)
• Publication Date: 2022-05-14
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2022/5783867
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Objective . Duchesnea indica has been reported for its anti-inflammatory properties. However, its efficacy in sepsis has yet to be reported. In this study, we studied the ability of Duchesnea indica extract (DIE) to rescue mice from septic shock and sepsis. Methods . In vitro studies included the measurement of secreted nitric oxide, cell viability, gene and protein expression via real-time polymerase chain reaction and western blot, and confocal microscopy in RAW 264.7 cells. In vivo studies include a model of septic shock and sepsis in BALB/c mice induced by a lethal and sub-lethal dose of lipopolysaccharide (LPS). Results . DIE suppressed the expression of proinflammatory cytokines induced by LPS and prevented the translocation of NF κ B into the nucleus of RAW 264.7 cells. It also prevented reactive oxygen species damage induced by LPS in murine bone marrow-derived macrophages. Models of sepsis and septic shock were established in BALB/ c mice and DIE-rescued mice from septic shock. DIE also reversed the increase in tumor necrosis factor- α and nitrite levels in the serum of mice induced with sepsis. DIE also prevented the translocation of NF κ B from the cytosol into the nucleus in murine lungs. Histopathological damage induced by sepsis was reversed in the testis, liver, and lungs of mice. Conclusion . In conclusion, DIE is a suitable candidate for development as a therapeutic agent for sepsis.