Exploring the complex map of insulin polymorphism: a novel crystalline form in the presence of m‐cresol. Issue 4 (7th April 2020)

• Record Type: Journal Article
• Authors: Karavassili, Fotini; Valmas, Alexandros; Dimarogona, Maria; Giannopoulou, Anastasia E.; Fili, Stavroula; Norrman, Mathias; Schluckebier, Gerd; Beckers, Detlef; Fitch, Andrew N.; Margiolaki, I.
• Is Part Of: Acta crystallographica. Volume 76:Issue 4(2020)
• Page Start: 366
• Page End: 374
• Publication Date: 2020-04-07
• Subjects: single‐crystal diffraction -- powder diffraction -- synchrotron radiation -- polymorphism -- human insulin -- m‐cresol -- diabetes mellitus; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; Molecular biology -- Periodicals; Molecular structure -- Periodicals; Biomolecules -- Structure -- Periodicals; Cytology -- Periodicals; Biomolecules -- Structure; Crystallography; Cytology; Molecular biology; Molecular structure; X-ray crystallography; Periodicals; 548
• DOI: 10.1107/S2059798320002545
• Languages: English
• ISSNs: 2059-7983

Abstract

Abstract : A novel monoclinic phase of human insulin co‐crystallized with m ‐cresol was structurally characterized by means of powder and single‐crystal X‐ray diffraction. Abstract : In this study, the first crystal structure of a novel crystal form of human insulin bound to meta ‐cresol in an acidic environment is reported. The combination of single‐crystal and powder X‐ray diffraction crystallography led to the detection of a previously unknown monoclinic phase ( P 21 ). The structure was identified from the powder patterns and was solved using single‐crystal diffraction data at 2.2 Å resolution. The unit‐cell parameters at pH 6.1 are a = 47.66, b = 70.36, c = 84.75 Å, β = 105.21°. The structure consists of two insulin hexamers per asymmetric unit. The potential use of this insulin form in microcrystalline drugs is discussed.