Preventive effects of nerve growth factor against colistin-induced autophagy and apoptosis in PC12 cells. (24th March 2019)

• Record Type: Journal Article
• Authors: Tian, Erjie; Hu, Wanjun; Miao, Yusong; Muhammad, Ishfaq; Zhang, Ling; Xia, Chunli; Ding, Liangjun; Zhang, Qiaomei; Li, Rui; Chen, Chunli; Li, Jichang
• Is Part Of: Toxicology mechanisms and methods. Volume 29:Number 3(2019)
• Page Start: 177
• Page End: 186
• Publication Date: 2019-03-24
• Subjects: NGF -- autophagy -- apoptosis -- colistin -- PC12 cells; Analytical toxicology -- Periodicals; Toxicology -- Periodicals; Toxicology -- Methodology -- Periodicals; 615.907
• DOI: 10.1080/15376516.2018.1534298
• Languages: English
• ISSNs: 1537-6516

Abstract

Abstract: In this study, the preventive effects of NGF against colistin-induced autophagy and apoptosis in PC12 cells have been investigated. Fluorescence microscopy, real-time PCR, transmission electron microscopy (TEM), flow cytometery, and western blotting technique were used. The results showed that large amounts of autophagosomes and apoptotic markers were triggered by colistin. Consistently, a significant increase has been noted at mRNA and protein levels in autophagy and apoptosis-related genes. Besides, TEM analysis showed that autophagic vacuoles were obvious at 12 h, while nuclear chromatin condensation and edge accumulation were clearly seen at 24 h in colistin alone group. Importantly, the visual autophagy and apoptosis were markedly reduced with NGF treatment in a dose-dependent manner. Moreover, colistin-induced reduction in mitochondrial membrane potential was partly attenuated by NGF in a dose dependent manner. In summary, NGF ameliorated colistin-induced apoptosis and autophagy, and partially recovered MMP in PC12 cells.