Lysophosphatidic acid acyltransferases: a link with intracellular protein trafficking in Arabidopsis root cells?. (19th November 2021)

• Record Type: Journal Article
• Authors: Wattelet-Boyer, Valérie; Le Guédard, Marina; Dittrich-Domergue, Franziska; Maneta-Peyret, Lilly; Kriechbaumer, Verena; Boutté, Yohann; Bessoule, Jean-Jacques; Moreau, Patrick
• Editors: Murphy, Angus
• Is Part Of: Journal of experimental botany. Volume 73:Number 5(2022)
• Page Start: 1327
• Page End: 1343
• Publication Date: 2021-11-19
• Subjects: Arabidopsis -- lysophosphatidic acid acyltransferase -- lysophosphatidic acid -- phosphatidic acid -- PIN2 -- PIP2;7 -- roots -- secretory pathway; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erab504
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract: Phosphatidic acid (PA) and lysophosphatidic acid acyltransferases (LPAATs) might be critical for the secretory pathway. Four extra-plastidial LPAATs (LPAAT2, 3, 4, and 5) were identified in Arabidopsis thaliana. These AtLPAATs display a specific enzymatic activity converting lysophosphatidic acid to PA and are located in the endomembrane system. We investigate a putative role for AtLPAATs 3, 4, and 5 in the secretory pathway of root cells through genetical (knockout mutants), biochemical (activity inhibitor, lipid analyses), and imaging (live and immuno-confocal microscopy) approaches. Treating a lpaat4;lpaat5 double mutant with the LPAAT inhibitor CI976 produced a significant decrease in primary root growth. The trafficking of the auxin transporter PIN2 was disturbed in this lpaat4;lpaat5 double mutant treated with CI976, whereas trafficking of H + -ATPases was unaffected. The lpaat4;lpaat5 double mutant is sensitive to salt stress, and the trafficking of the aquaporin PIP2;7 to the plasma membrane in the lpaat4;lpaat5 double mutant treated with CI976 was reduced. We measured the amounts of neo-synthesized PA in roots, and found a decrease in PA only in the lpaat4;lpaat5 double mutant treated with CI976, suggesting that the protein trafficking impairment was due to a critical PA concentration threshold. Abstract : Phosphatidic acid produced by lysophosphatidic acid acyltransferases has an impact on the efficiency of the intracellular trafficking of some proteins in Arabidopsis thaliana root cells.