Toxic effects of dechlorane plus on the common carp (Cyprinus carpio) embryonic development. (June 2020)

• Record Type: Journal Article
• Authors: Li, Baohua; Chen, Jianjun; Du, Qiyan; Wang, Beibei; Qu, Ying; Chang, Zhongjie
• Is Part Of: Chemosphere. Volume 249(2020)
• Publication Date: 2020-06
• Subjects: Dechlorane plus -- Common carps -- Embryo -- Developmental toxicity -- DNA damage; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2020.126481
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Dechlorane Plus (DP) is a widely used chlorinated flame retardant, which has been extensively detected in the environment. Although DP content in the surface water is low, it can pose a continuous exposure risk to aquatic organisms due to its strong bioaccumulation. Considering that the related studies on the toxicity mechanism of DP exposure are limited, the effect of DP on carp embryo development was evaluated. In the present work, carp embryos were exposed to different concentrations (0, 30, 60, and 120 μg/L) of DP at 3 h post-fertilization (hpf). The expression levels of neural and skeletal development-associated genes, such as sox2, sox19a, Mef2c and BMP4, were detected with quantitative PCR, and the changes in different developmental toxicity endpoints were observed. Our results demonstrated that the expression levels of sox2, sox19a, Mef2c and BMP4 were significantly altered and several developmental abnormalities were found in DP-exposed carp embryos, such as DNA damage, increased mortality rate, delayed hatching time, reduced hatching rate, decreased body length, and increased morphological deformities. In addition, the activities of reactive oxygen species and malondialdehyde were remarkably higher in 60 and 120 μg/L DP exposure groups than in control group. These results suggest that DP can exhibit a unique modes of action, which lead to aberration occurrence in the early development stage of common carps, which may be related to some gene damage and oxidative stress. Besides, the parameters evaluated here can be used as tools to access the environmental risk for biota and humans exposed to DP. Graphical abstract: Image 1 Highlights: DP has embryotoxic effects on common carp. DP could delay embryo development and even cause embryo death. DP exposure could induce DNA damage and oxidative stress. ROS and MDA may play an important role in DP-induced developmental deformity.