PDS5 proteins regulate the length of axial elements and telomere integrity during male mouse meiosis. (14th April 2020)

• Record Type: Journal Article
• Authors: Viera, Alberto; Berenguer, Inés; Ruiz‐Torres, Miguel; Gómez, Rocío; Guajardo, Andrea; Barbero, José Luis; Losada, Ana; Suja, José A
• Is Part Of: EMBO reports. Volume 21:Number 6(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-04-14
• Subjects: axial elements -- meiosis -- mouse -- PDS5 -- telomeres; Molecular biology -- Periodicals; Molecular Biology -- Periodicals; Molecular biology; Periodicals; 572.8
• DOI: 10.15252/embr.201949273
• Languages: English
• ISSNs: 1469-221X

Abstract

Abstract: Cohesin cofactors regulate the loading, maintenance, and release of cohesin complexes from chromosomes during mitosis but little is known on their role during vertebrate meiosis. One such cofactor is PDS5, which exists as two paralogs in somatic and germline cells, PDS5A and PDS5B, with unclear functions. Here, we have analyzed their distribution and functions in mouse spermatocytes. We show that simultaneous excision of Pds5A and Pds5B results in severe defects during early prophase I while their individual depletion does not, suggesting their functional redundancy. Shortened axial/lateral elements and a reduction of early recombination nodules are observed after the strong depletion of PDS5A/B proteins. Moreover, telomere integrity and their association to the nuclear envelope are severely compromised. As these defects occur without detectable reduction in chromosome‐bound cohesin, we propose that the dynamic behavior of the complex, mediated by PDS5 proteins, is key for successful completion of meiotic prophase I. Synopsis: Analysis of spermatogenesis in mice carrying conditional knock out alleles for cohesin cofactors PDS5A and PDS5B reveals the importance of cohesin dynamics for proper assembly of the synaptonemal complex, homolog recombination and telomere integrity. A single PDS5 protein is sufficient to complete prophase I successfully. Shortened axial/lateral elements and a reduction of early recombination nodules are observed after simultaneous depletion of the two PDS5 proteins without detectable reduction in cohesin levels. PDS5 proteins ensure the integrity of telomeres and their attachment to the nuclear envelope. Abstract : Analysis of spermatogenesis in mice carrying conditional knock out alleles for cohesin cofactors PDS5A and PDS5B reveals the importance of cohesin dynamics for proper assembly of the synaptonemal complex, homolog recombination and telomere integrity.