Coining attributes of ultra-low concentration graphene oxide and spermine: An approach for high strength, anti-microbial and osteoconductive nanohybrid scaffold for bone tissue regeneration. (January 2019)

• Record Type: Journal Article
• Authors: Ghorai, Sanjoy Kumar; Maji, Somnath; Subramanian, Bhuvaneshwaran; Maiti, Tapas Kumar; Chattopadhyay, Santanu
• Is Part Of: Carbon. Volume 141(2019)
• Page Start: 370
• Page End: 389
• Publication Date: 2019-01
• Subjects: Carbon -- Periodicals; Carbone -- Périodiques; Koolstof; Toepassingen; Electronic journals; 546.681
• DOI: 10.1016/j.carbon.2018.09.062
• Languages: English
• ISSNs: 0008-6223

Abstract

Abstract: Lack of auto osteogenesis and clinical complexities of repairing of bone after severe injuries, urge to develop a significant approach to promote regeneration of bone. The present work represents ornamentation of 2D rod-like nanohydroxyapatite (nHA) on ultra-low concentration graphene oxide (GO) sheet. The nanohybrids (GO-nHA) were incorporated into the spermine based high strength thermoplastic polyurethane-urea (PUU) matrices by in situ technique and the porous scaffolds were fabricated. 1 wt% GO-nHA filled scaffold displayed dramatically improved physico-mechanical properties. Cytotoxicity study using osteoblast cell like MG-63 cell line revealed positive cell viability (above 95%) and increased proliferation over a period of 14 days of culture. Semi-quantitative Real Time polymerase Chain Reaction (qRT-PCR) showed positive expression of collagen type I and osteocalcin indicating excellent maturation and biomineralization of osteoblasts. To avoid burden of carbonaceous particulate in body, very low percentage of GO (0.15%) was incorporated into the scaffold to improve mechanical properties, surface wettability, cell viability and cell proliferation drastically. Furthermore, in vivo study confirmed the promoted osteogenesis of PUU/GO-nHA scaffold in vivo without any cytotoxicity. Based on mechanical, in vitro and in vivo study the high strength nanohybrid scaffold exhibited tremendous potential for orthopedic applications. Graphical abstract: Image 1