Protein‐Recognition‐Initiated Exponential Amplification Reaction (PRIEAR) and Its Application in Clinical Diagnosis. (2nd February 2022)

• Record Type: Journal Article
• Authors: Hu, Changjia; Zhang, Jie; Jin, Yanwen; Ma, Wenjie; Zhou, Rongxing; Du, Huan; Yang, Peng; Hou, Xiandeng; Cheng, Nansheng; Chen, Junbo
• Is Part Of: Chembiochem. Volume 23:Number 6(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-02
• Subjects: analytical methods -- clinical diagnosis -- exponential amplification -- homogeneous -- protein recognition; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202100548
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Isothermal exponential amplification technology has rarely been fabricated as a universal sensing platform for the detection of proteins. To broaden their application, we have developed a strategy, named protein‐recognition‐initiated exponential amplification reaction (PRIEAR) using protein recognition to induce DNA assembly, which converts protein recognition events into ssDNA amplicons and combines two‐stage amplification to achieve exponential amplification. Taking advantage of this principle, diverse biomarkers can be quantified at sub‐picomolar concentrations in a homogenous manner, making PRIEAR suitable for clinical settings. Therefore, this strategy can expand the powerful isothermal exponential amplification technology to protein targets and thus provide a new toolbox in clinical and biomedical applications. Abstract : Protein‐recognition‐initiated exponential amplification reaction (PRIEAR) using protein recognition to induce DNA assembly is reported. This technology converts protein recognition events into ssDNA amplicons and combines two‐stage amplification to achieve exponential amplification. Diverse biomarkers can be quantified at sub‐picomolar concentrations by using PRIEAR.