Evaluation of the Solus One Listeria Method for the Detection of Listeria Species on Environmental Surfaces. (23rd November 2019)

• Record Type: Journal Article
• Authors: Tonner, Elizabeth; Kelly, Siobhan; Illingworth, Simon; Perera, Nevin; Bastin, Benjamin; Bird, Patrick; Joseph Benzinger, M; Agin, James; Goins, David; Chen, Yi; Salfinger, Yvonne; Brodsky, Michael
• Is Part Of: Journal of AOAC International. Volume 102:Number 2(2019)
• Page Start: 570
• Page End: 579
• Publication Date: 2019-11-23
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.18-0199
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: Background: Solus One Listeria is designed to accurately detect Listeria species ( Listeria grayi, L. innocua, L. ivanovii, L. marthii, L. monocytogenes, L. seeligeri, and L. welshimeri ) from stainless steel and plastic environmental surface matrixes using an antibody-based technology test system paired with proprietary SOLO+ media and combined with manual or automated sample preparation method. Objective: Solus One Listeria was evaluated for inclusivity and exclusivity and a matrix comparison study fortwo environmental surfaces. Methods: Solus One Listeria was comparedwith the following reference method for the method comparison study: the U.S. Food and Drug Administration Bacteriological Analytical Manual Chapter 10 from stainless steel and plastic environmental surfaces. Both the manual and automatedpreparation methods were performed for stainless steel and plastic environmental surfaces. Results: For the inclusivity and exclusivityevaluation, Solus One Listeria correctly identified all 50 target organism isolates and correctly excluded all 30 nontarget strains that were analyzed. In the method comparison study, bothSolus One Listeria manual and automated preparation methods demonstrated no significant differences based on probability of detection statistical analysis between presumptive and confirmed results or between candidate and reference methodresults for two environmental surfaces after 22–30 h of enrichment time. Probability of detection analysis of Solus One Listeria method robustness, product consistency (lot-to-lot), and stability studies using the automated preparation method demonstrated no statistically significant differences. Conclusions: The data from the study support the product claims of Solus One Listeria for the accurate detection of Listeria species, using both the manual and automated methods (using theDynex DS2 instrument), on both environmental surfaces analyzed.