J‐Aggregate‐Based FRET Monitoring of Drug Release from Polymer Nanoparticles with High Drug Loading. Issue 45 (15th September 2020)

• Record Type: Journal Article
• Authors: Liu, Yun; Yang, Guangze; Jin, Song; Zhang, Run; Chen, Peng; Tengjisi, ; Wang, Lianzhou; Chen, Dong; Weitz, David A.; Zhao, Chun‐Xia
• Is Part Of: Angewandte Chemie international edition. Volume 59:Issue 45(2020)
• Page Start: 20065
• Page End: 20074
• Publication Date: 2020-09-15
• Subjects: drug release -- FRET -- J-aggregates -- nanoparticles -- polymers; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202008018
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Understanding drug‐release kinetics is critical for the development of drug‐loaded nanoparticles. We developed a J‐aggregate‐based Förster‐resonance energy‐transfer (FRET) method to investigate the release of novel high‐drug‐loading (50 wt %) nanoparticles in comparison with low‐drug‐loading (0.5 wt %) nanoparticles. Single‐dye‐loaded nanoparticles form J‐aggregates because of the high dye‐loading (50 wt %), resulting in a large red‐shift (≈110 nm) in the fluorescence spectrum. Dual‐dye‐loaded nanoparticles with high dye‐loading using FRET pairs exhibited not only FRET but also a J‐aggregate red‐shift (116 nm). Using this J‐aggregate‐based FRET method, dye‐core–polymer‐shell nanoparticles showed two release processes intracellularly: the dissolution of the dye aggregates into dye molecules and the release of the dye molecules from the polymer shell. Also, the high‐dye‐loading nanoparticles (50 wt %) exhibited a slow release kinetics in serum and relatively quick release in cells, demonstrating their great potential in drug delivery. Abstract : A J‐aggregate‐based Förster‐resonance energy‐transfer (FRET) method to investigate the release of high‐drug‐loading (50 wt %) and low‐drug‐loading (0.5 wt %) nanoparticles is reported. The high‐dye‐loading nanoparticles exhibited a slow release kinetics in serum and a relatively quick release in cells, demonstrating their great potential in drug delivery.