Single‐cell multi‐omics analysis presents the landscape of peripheral blood T‐cell subsets in human chronic prostatitis/chronic pelvic pain syndrome. Issue 23 (30th October 2020)

• Record Type: Journal Article
• Authors: Zhang, Meng; Liu, Yi; Chen, Junyi; Chen, Lei; Meng, Jialin; Yang, Cheng; Yin, Shuiping; Zhang, Xiansheng; Zhang, Li; Hao, Zongyao; Chen, Xianguo; Liang, Chaozhao
• Is Part Of: Journal of cellular and molecular medicine. Volume 24:Issue 23(2020)
• Page Start: 14099
• Page End: 14109
• Publication Date: 2020-10-30
• Subjects: chronic prostatitis/chronic pelvic pain syndrome -- effector T cell -- single‐cell multi‐omics -- T regular cell; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.16021
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: Cumulative evidence suggests that abnormal differentiation of T lymphocytes influences the pathogenesis of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Thus, understanding the immune activation landscape of CP/CPPS would be helpful for improving therapeutic strategies. Here, we utilized BD™ AbSeq to digitally quantify both the protein and mRNA expression levels in single peripheral blood T cells from two CP/CPPS patients and two healthy controls. We utilized an integrated strategy based on canonical correlation analysis of 10 000+ AbSeq profiles and identified fifteen unique T‐cell subpopulations. Notably, we found that the proportion of cluster 0 in the CP/CPPS group (30.35%) was significantly increased compared with the proportion in the healthy control group (9.38%); cluster 0 was defined as effector T cells based on differentially expressed genes/proteins. Flow cytometry assays confirmed that the proportions of effector T‐cell subpopulations, particularly central memory T cells, T helper (Th)1, Th17 and Th22 cells, in the peripheral blood mononuclear cell populations of patients with CP/CPPS were significantly increased compared with those of healthy controls ( P < 0.05), further confirming that aberration of effector T cells possibly leads to or intensifies CP/CPPS. Our results provide novel insights into the underlying mechanisms of CP/CPPS, which will be beneficial for its treatment.