Acinetobacter lanii sp. nov., Acinetobacter shaoyimingii sp. nov. and Acinetobacter wanghuae sp. nov., isolated from faeces of Equus kiang. Issue 1 (16th January 2021)

• Record Type: Journal Article
• Authors: Zhu, Wentao; Dong, Kui; Yang, Jing; Lu, Shan; Lai, Xin-He; Pu, Ji; Jin, Dong; Huang, Yuyuan; Zhang, Sihui; Zhou, Juan; Huang, Ying; Xu, Jianguo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 1(2021)
• Publication Date: 2021-01-16
• Subjects: Acinetobacter lanii -- Acinetobacter shaoyimingii -- Acinetobacter wanghuae -- Equus kiang -- Qinghai–Tibet Plateau; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004567
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Six aerobic, non-motile, non-haemolytic, Gram-stain-negative, oxidase-negative strains (185 T, 187, 323-1 T, 194, dk386 T and dk771) were recovered from different faecal samples of Equus kiang on the Qinghai–Tibet Plateau. In the 16S rRNA gene sequences, one strain pair, 185 T /187, shared highest similarity to Acinetobacter equi 114 T (97.9 %), and the other two (323-1 T /194 and dk771 T /dk386) to Acinetobacter harbinensis CGMCC 1.12528 T (98.6 and 97.0 %, respectively). Phylogenomic tree analysis showed that these six strains formed three separate clades in the genus Acinetobacter . Digital DNA–DNA hybridization values of each pair of the isolates with all members of the genus Acinetobacter were far below 70 %. The main cellular fatty acids of all six strains were C18 : 1 ω 9 c, C16 : 0 and summed feature 3 (C16 : 1 ω 7 c /C16 : 1 ω 6 c ). Q-9 was the predominant respiratory quinone for strains 185 T, 323-1 T and dk386 T . The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the genotypic, phenotypic and biochemical analyses, these six strains represent three novel species of the genus Acinetobacter, for which the names Acinetobacter lanii sp. nov., Acinetobacter shaoyimingii sp. nov. and Acinetobacter wanghuae sp. nov. are proposed. The type strains are 185 T (=CGMCC 1.13636 T =JCM 33607 T ), 323-1 T (=CGMCC 1.13940 T =JCM 33608 T ) and dk386 T (=CGMCC 1.16589 T =JCM 33592 T ), respectively.