A Deep Learning Pipeline for Nucleus Segmentation. Issue 12 (19th November 2020)

• Record Type: Journal Article
• Authors: Zaki, George; Gudla, Prabhakar R.; Lee, Kyunghun; Kim, Justin; Ozbun, Laurent; Shachar, Sigal; Gadkari, Manasi; Sun, Jing; Fraser, Iain D. C.; Franco, Luis M.; Misteli, Tom; Pegoraro, Gianluca
• Is Part Of: Cytometry. Volume 97:Issue 12(2020)
• Page Start: 1248
• Page End: 1264
• Publication Date: 2020-11-19
• Subjects: deep learning -- machine learning -- nucleus segmentation -- fluorescence microscopy -- high‐content imaging; Flow cytometry -- Periodicals; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnostic imaging -- Periodicals; 571.605
• DOI: 10.1002/cyto.a.24257
• Languages: English
• ISSNs: 1552-4922

Abstract

Abstract: Deep learning is rapidly becoming the technique of choice for automated segmentation of nuclei in biological image analysis workflows. In order to evaluate the feasibility of training nuclear segmentation models on small, custom annotated image datasets that have been augmented, we have designed a computational pipeline to systematically compare different nuclear segmentation model architectures and model training strategies. Using this approach, we demonstrate that transfer learning and tuning of training parameters, such as the composition, size, and preprocessing of the training image dataset, can lead to robust nuclear segmentation models, which match, and often exceed, the performance of existing, off‐the‐shelf deep learning models pretrained on large image datasets. We envision a practical scenario where deep learning nuclear segmentation models trained in this way can be shared across a laboratory, facility, or institution, and continuously improved by training them on progressively larger and varied image datasets. Our work provides computational tools and a practical framework for deep learning‐based biological image segmentation using small annotated image datasets. Published [2020]. This article is a U.S. Government work and is in the public domain in the USA