Is there any correlation between sperm parameters and chromatin quality with embryo morphokinetics in patients with male infertility?. (12th March 2018)

• Record Type: Journal Article
• Authors: Mangoli, E.; Khalili, M. A.; Talebi, A. R.; Ghasemi‐Esmailabad, S.; Hosseini, A.
• Is Part Of: Andrologia. Volume 50:Number 5(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-03-12
• Subjects: chromatin -- embryo kinetics -- intracytoplasmic sperm injection -- sperm parameters -- time lapse; Andrology -- Periodicals; Infertility, Male -- Periodicals; 616.69
• DOI: 10.1111/and.12997
• Languages: English
• ISSNs: 0303-4569

Abstract

Summary: The main goal was to evaluate the correlation between sperm parameters and chromatin quality with embryo kinetics via time‐lapse monitoring system (TLM). A total of 40 couples involved in the ICSI program as a result of male infertility. For assessment of sperm chromatin and DNA quality, we used aniline blue, toluidine blue, chromomycin A3, acridine orange and terminal transferase‐mediated deoxyuridine triphosphate biotin end labelling assays. All mature oocytes were injected, and the generated zygotes (2PNs) were cultured in TLM. In day 3 after injection, single embryo transfer (SET) was carried out according to the morphology and morphokinetics. The patients were followed up until delivery. There were positive significant correlations between sperm count with CC2 ( r = .330, p = .049), T4 ( r = .329, p = .038), T6 ( r = .342, p = .035) and T7 ( r = .374, p = .025). Also, there were positive significant correlations between nonprogressive motility and T2 (r = 0.323, p = .042), T3 ( r = .411, p = .013) and T4 ( r = .418, p = .007). Regarding the sperm chromatin quality assays, there were negative significant correlations between CMA3 and CC2 ( r = −.272, p = .049) and between acridine orange and T5 ( r = −.221, p = .040). It seems that the abnormal sperm parameters and chromatin alteration affect the normal embryo kinetics in ICSI program.