A Novel Fluorescent Probe for Detection of Hydrogen Sulfide and Its Bioimaging Applications in Living Cells. Issue 2 (16th January 2020)

• Record Type: Journal Article
• Authors: Zhu, Lei; Liao, Wenhao; Chang, Haizhen; Liu, Xianjun; Miao, Shaobin
• Is Part Of: ChemistrySelect. Volume 5:Issue 2(2020)
• Page Start: 829
• Page End: 833
• Publication Date: 2020-01-16
• Subjects: Azide -- fluorescent probe -- hydrogen sulfide -- lysosome -- self-immolative group; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201903451
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Hydrogen sulfide (H2 S) is a critical gasotransmitter playing an important role in various physiological and pathological processes. The development of fluorescent probes for rapid, sensitive, and selective lysosomal H2 S sensing has attracted much attention. Herein we report a rhodamine based probe MF‐N3 that can selectively accumulate in lysosomes and give turn‐on fluorescence in the presence of both H2 S and protons. Results show that MF‐N3 has an exclusive response to H2 S in the acidic lysosomal environment but does not respond in other organelles or in neutral environments. This strategy can circumvent the fluorescence interference from H2 S of other sources, thus providing a high sensitivity and selectivity on lysosomal H2 S imaging by fluorescence microscopy. This pH‐dependent lysosome‐targeting fluorescent probe has been successfully applied to lysosomal H2 S imaging in living cells and is of great potential for applications to the environmental and biological systems. Abstract : A small molecule probe displays turn‐on fluorescence in the presence of hydrogen sulfide under acidic conditions. It has good selectivity for hydrogen sulfide with a low detection limit. The probe can accumulate exclusively in lysosomes and, thus, can be successfully applied to fluorescence imaging of hydrogen sulfide in living cells. The sensing mechanism was proposed and analyzed by HPLC and mass spectrometry.