Proteomic analysis reveals dynamic regulation of fruit development and sugar and acid accumulation in apple. (17th August 2016)

• Record Type: Journal Article
• Authors: Li, Mingjun; Li, Dongxia; Feng, Fengjuan; Zhang, Sheng; Ma, Fengwang; Cheng, Lailiang
• Is Part Of: Journal of experimental botany. Volume 67:Number 17(2016)
• Page Start: 5145
• Page End: 5157
• Publication Date: 2016-08-17
• Subjects: Amino acid -- apple -- fruit development -- functional annotation -- organic acid -- sugar -- tandem mass tag.; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erw277
• Languages: English
• ISSNs: 0022-0957

Abstract

Highlight: Proteomic analysis of apple fruit development provided a comprehensive view of the major processes and pathways underlying apple fruit development and key fruit quality traits. Abstract: Understanding the fruit developmental process is critical for fruit quality improvement. Here, we report a comprehensive proteomic analysis of apple fruit development over five growth stages, from young fruit to maturity, coupled with metabolomic profiling. A tandem mass tag (TMT)-based comparative proteomics approach led to the identification and quantification of 7098 and 6247 proteins, respectively. This large-scale proteomic dataset presents a global view of the critical pathways involved in fruit development and metabolism. When linked with metabolomics data, these results provide new insights into the modulation of fruit development, the metabolism and storage of sugars and organic acids (mainly malate), and events within the energy-related pathways for respiration and glycolysis. We suggest that the key steps identified here (e.g. those involving the FK2, TST, EDR6, SPS, mtME and mtMDH switches), can be further targeted to confirm their roles in accumulation and balance of fructose, sucrose and malate. Moreover, our findings imply that the primary reason for decreases in amino acid concentrations during fruit development is related to a reduction in substrate flux via glycolysis, which is mainly regulated by fructose-bisphosphate aldolase and bisphosphoglycerate mutase.