Basics of Digital Microscopy. Issue 1 (18th January 2018)

• Record Type: Journal Article
• Authors: Wallace, Callen T.; Jessup, Morgan; Bernas, Tytus; Peña, Karina A.; Calderon, Michael J.; Loughran, Patricia A.
• Editors: Robinson, J. Paul; Darzynkiewicz, Zbigniew; Hoffman, Robert; Nolan, John P.; Rabinovitch, Peter S.; Watkins, Simon
• Is Part Of: Current protocols in cytometry. Volume 83:Issue 1(2018)
• Page Start: 12.2.1
• Page End: 12.2.14
• Publication Date: 2018-01-18
• Subjects: image file format -- quantization -- resolution -- sampling -- signal‐to‐noise ratio -- compression; Cytology -- Laboratory manuals; Flow cytometry -- Laboratory manuals; Cell separation -- Laboratory manuals; Molecular biology -- Laboratory manuals; Flow Cytometry -- methods; Image Cytometry -- methods; Cell Separation -- methods; Cytological Techniques; Molecular Biology -- methods; Cell separation; Cytology; Flow cytometry; Molecular biology; Laboratory Manuals; Laboratory manuals; 571.6
• DOI: 10.1002/cpcy.31
• Languages: English
• ISSNs: 1934-9297

Abstract

Abstract: Modern digital microscopy combines the equipment of classical light microscopy with a computerized imaging system. The technique comprises image formation by optics, image registration by a camera, and saving of image data in a computer file. This chapter describes limitations that are particular to each of these processes, including optical resolution, efficiency of image registration, characteristics of image file formats, and data management. Further suggestions are given which serve, in turn, to help construct a set of guidelines aimed at optimization of digital microscopic imaging. © 2018 by John Wiley & Sons, Inc.