Overview of qPCR Molecular Probes. Issue 11 (2nd November 2015)

• Record Type: Journal Article
• Authors: Laikhter, Andrei
• Editors: Gallagher, Sean R.; Wiley, Emily A.
• Is Part Of: Current protocols essential laboratory techniques. Issue 11(2015)
• Page Start: 10.5.1
• Page End: 10.5.10
• Publication Date: 2015-11-02
• Subjects: qPCR -- fluorophore -- quencher dye -- molecular probes -- molecular beacons -- fluorescence resonance energy transfer (FRET); Life sciences -- Laboratory manuals; Medical laboratories; Diagnosis, Laboratory; Clinical Laboratory Techniques; Clinical Protocols; Diagnosis, Laboratory; Medical laboratories; 570.28
• DOI: 10.1002/9780470089941.et1005s11
• Languages: English
• ISSNs: 1948-3430

Abstract

Abstract: In recent times, varieties of modified synthetic oligonucleotides conjugated with reporter molecules have been employed in hybridization‐based assays. These methods have broad applications in both basic molecular biology research and in clinical diagnostics and screening because they have high specificity to a target nucleic acid sequence. The development of the fluorescence resonance energy transfer (FRET) process has improved the performance and utility of these hybridization‐based assays. In this unit, we discuss the main principles of the design and synthesis of the qPCR probes. © 2015 by John Wiley & Sons, Inc.