Antibody-conjugated liposomes loaded with indocyanine green for oral targeted photoacoustic imaging-guided sonodynamic therapy of Helicobacter pylori infection. (15th April 2022)

• Record Type: Journal Article
• Authors: Wang, Ruhao; Song, Cunfeng; Gao, Ang; Liu, Qianwen; Guan, Wenbin; Mei, Jiawei; Ma, Lijun; Cui, Daxiang
• Is Part Of: Acta biomaterialia. Volume 143(2022)
• Page Start: 418
• Page End: 427
• Publication Date: 2022-04-15
• Subjects: Helicobacter pylori -- Sonodynamic therapy -- Indocyanine green -- Photoacoustic imaging; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2022.02.031
• Languages: English
• ISSNs: 1742-7061

Abstract

Abstract: Helicobacter pylori is a causative factor of various gastrointestinal tract diseases. As clinical antibiotic-based therapy for H. pylori infection might induce bacterial drug resistance, the in vivo eradication of H. pylori remains a huge challenge. In the present study, monoclonal antibody-conjugated liposomes loaded with indocyanine green (ICG) ( Hp Ab-LiP-ICG) were successfully developed for targeted photoacoustic (PA) imaging-guided sonodynamic therapy (SDT) of H. pylori infection in vivo. Hp Ab-LiP-ICG showed high stability and favorable biocompatibility in acidic environment (pH 1.5) and was used for treating H. pylori- infected mice through oral administration. PA imaging showed that Hp Ab-LiP-ICG could precisely recognize and target H. pylori in the stomach. Following the targeting of Hp Ab-LiP-ICG to H. pylori, ICG was activated to generate singlet oxygen ( 1 O2 ) for eliminating H. pylori under ultrasound (US) irradiation. Pathological analysis revealed that the Hp Ab-LiP-ICG-mediated SDT eradicated H. pylori without unintended toxicity to normal tissues. In conclusion, the Hp Ab-LiP-ICG-mediated SDT might shed new light on treating H. pylori infection, indicating the clinical translational prospects of this therapy in near future. Statement of significance: Traditional antibiotic-based therapy for Helicobacter pylori infections suffers from the risk of drug resistance. To meet this challenge, a monoclonal antibody-conjugated nanoliposome loaded with indocyanine green (ICG) ( Hp Ab-LiP-ICG) was successfully developed, and efficient eradication of H. pylori was achieved in vivo by visual sonodynamic therapy (SDT). Hp Ab-LiP-ICG exhibited biocompatibility, targeting, and stability in the acidic microenvironment. Under ultrasound (US) irradiation in vitro, the Hp Ab-LiP-ICG nanoliposomes accumulated on the surface of H. pylori were activated to produce adequate singlet oxygen ( 1 O2 ) to eliminate H. pylori. Gastric mucous tissues infected with H. pylori recovered to the normal state after Hp Ab-LiP-ICG-mediated SDT without side effects, thus highlighting the clinical translational prospects of the prepared Hp Ab-LiP-ICG nanoliposome in near future. Graphical abstract: Image, graphical abstract