Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/Akt/NOX4. (May 2022)

• Record Type: Journal Article
• Authors: Ma, Chuang; Lyu, Mingyue; Deng, Chilu; Liu, Xia; Cui, Yanmin; Shen, Yixiao; Meng, Xianjun; Zhao, Jin
• Is Part Of: Journal of functional foods. Volume 92(2022)
• Publication Date: 2022-05
• Subjects: Aronia melanocarpa -- Cyanidin-3-galactoside -- Pulmonary fibrosis -- Fibroblast differentiation -- Oxidative stress; A. melanocarpa Aronia melanocarpa -- C3G cyanidin-3-galactoside -- SP silica particle -- NOX4 NADPH oxidase 4 -- Akt protein kinase B -- HPLC high performance liquid chromatography -- UPLC ultra-performance liquid chromatography -- IHC immunohistochemistry -- H&E hematoxylin and eosin -- TGF-β Transforming growth factor-β -- IgG immunoglobulin G -- HRP horseradish peroxidase -- DAB 3, 3′-diaminobenzidine -- RT-qPCR quantitative real-time polymerase chain reaction -- GAPDH glyceraldehyde-phosphate dehydrogenase -- ELISA commercial enzyme-linked immunosorbent assay -- α-SMA α-smooth muscle actin -- Col-1 Collagen 1 -- MDA malondialdehyde -- SOD superoxide dismutase -- GSH-Px glutathione peroxidase -- SDS-PAGE sodium dodecyl salt-polyacrylamide gel electrophoresis -- PVDF polyvinylidene fluoride -- TBST Tris-buffered saline containing Tween 20 -- NRF2 nuclear factor (erythroid-derived 2)-like 2 -- MAPK mitogen-activated protein kinase; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105034
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: C3G, the most abundant anthocyanin in A. melanocarpa, exerts beneficial health effects. C3G ameliorated SP-induced pulmonary fibrosis, inhibited fibroblasts activation, and downregulated oxidative stress levels. The mechanism involves the Nrf2, p38, Akt, NOX4 signaling pathways. Abstract: Aronia melanocarpa is rich in anthocyanins with many beneficial effects. Cyanidin-3-galactoside (C3G), the most abundant anthocyanin in Aronia melanocarpa was extracted and applied to silica particle-induced (SP-induced) pulmonary fibrosis mice and transforming growth factor-β-induced differentiation of NIH3T3 fibroblasts cell line to investigate the anti-fibrotic properties and underlying cellular mechanisms. The administration of C3G inhibited fibroblast differentiation through antioxidant effects, thereby reducing SP-induced pulmonary fibrosis. In vivo experiments showed that C3G reduced the fibrous nodules and collagen deposition in the lungs, the level of fibroblast markers, and the secretion of pro-fibrotic factors. C3G can also maintain the balance of oxidation and antioxidant in the lungs of mice, inhibit the phosphorylation of mitogen-activated protein kinase p38 and protein kinase B, down-regulate the level of NADPH oxidase 4 and increase the level of nuclear factor (erythroid-derived 2) like 2 express. The in vitro mechanism study of NIH3T3 cells verified the results of in vivo experiments. These findings emphasized the potential use of C3G as a functional food to alleviate SP-induced pulmonary fibrosis.