Catheterization of Ventral Caudal Artery in Rats: A Modified Technique for Repeatable Angiography and Intervention. Issue 5 (4th May 2022)

• Record Type: Journal Article
• Authors: Gao, Qun; Fan, Jingwen; Hu, Shen; Wang, Daming
• Is Part Of: Journal of investigative surgery. Volume 35:Issue 5(2022)
• Page Start: 1083
• Page End: 1090
• Publication Date: 2022-05-04
• Subjects: Catheterization technique -- caudal ventral artery -- angiography -- interventional operation -- animal model; Surgery -- Research -- Periodicals; Research; Surgery; Surgical Procedures, Operative; 617.075
• DOI: 10.1080/08941939.2021.2011493
• Languages: English
• ISSNs: 0894-1939

Abstract

Abstract: Background: The use of digital silhouette angiography (DSA) has been restricted due to lack of a technique that allows for repeated intra-arterial inspections over a prolonged period. Current studies are focused on the arteries that can be cannulated multiple times. We intended to develop and test a technique that would enable repeated performance of various catheterizations and transcatheter operations for a prolonged period, at the same site, with fewer postsurgical complications. Methods: Thirty rats were randomly divided into five groups. Ventral caudal artery cannulation was performed via the transtail approach after grouping for subsequent experiments. Histological staining and scanning electron microscopy were used to assess endothelial injuries. Results: The rats survived post catheterization of ventral caudal artery and establishment of animal models. The average time of ventral caudal artery cannulation was significantly shorter than that of the femoral (p < 0.01) and common carotid arteries (p < 0.01). In rats, the transtail artery technique effectively allowed selective arterial catheterization and angiography. Histological staining and scanning electron microscopy of the abdominal aorta revealed disruption of the intima and denuded wavy endothelial surface. Conclusions: We describe a novel method for artery sheath catheterization through the ventral caudal artery in rats; it may be possible to perform serial DSA studies and interventional operations with a single sheath channel in rats over a prolonged period. We believe that this approach will improve the utility of rats as models of human diseases and enable the broader use of rodent models for endovascular therapy research.