PEGylated liposomes associate with Wnt3A protein and expand putative stem cells in human bone marrow populations. (April 2017)

• Record Type: Journal Article
• Authors: Janeczek, Agnieszka A; Scarpa, Edoardo; Horrocks, Mathew H; Tare, Rahul S; Rowland, Caroline A; Jenner, Dominic; Newman, Tracey A; Oreffo, Richard OC; Lee, Steven F; Evans, Nicholas D
• Is Part Of: Nanomedicine. Volume 12:Number 8(2017)
• Page Start: 845
• Page End: 863
• Publication Date: 2017-04
• Subjects: fracture repair -- liposomes -- marrow stromal cells -- mesenchymal stem cells -- osteoprogenitors -- PEGylated liposomes -- regenerative medicine -- skeletal stem cells -- Wnt; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0386
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To fabricate PEGylated liposomes which preserve the activity of hydrophobic Wnt3A protein, and to demonstrate their efficacy in promoting expansion of osteoprogenitors from human bone marrow.Methods: PEGylated liposomes composed of several synthetic lipids were tested for their ability to preserve Wnt3A activity in reporter and differentiation assays. Single-molecule microspectroscopy was used to test for direct association of protein with liposomes.Results: Labeled Wnt3A protein directly associated with all tested liposome preparations. However, Wnt3A activity was preserved or enhanced in PEGylated 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes but not in PEGylated 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes. PEGylated Wnt3A liposomes associated with skeletal stem cell populations in human bone marrow and promoted osteogenesis.Conclusion: Active Wnt protein-containing PEGylated liposomes may have utility for systemic administration for bone repair.