A semiautomated microfluidic platform for real-time investigation of nanoparticles' cellular uptake and cancer cells' tracking. (March 2017)

• Record Type: Journal Article
• Authors: Carvalho, Mariana R; Maia, F Raquel; Silva-Correia, Joana; Costa, Bruno M; Reis, Rui L; Oliveira, Joaquim M
• Is Part Of: Nanomedicine. Volume 12:Number 6(2017)
• Page Start: 581
• Page End: 596
• Publication Date: 2017-03
• Subjects: cell tracking -- circulating tumor cells -- microfluidics -- nanoparticles -- theranostics; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0344
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Develop a platform composed of labeled dendrimer nanoparticles (NPs) and a microfluidic device for real-time monitoring of cancer cells fate.Materials & methods: Carboxymethylchitosan/poly(amidoamine) dendrimer NPs were labeled with fluorescein-5(6)-isothiocyanate and characterized using different physicochemical techniques. After, HeLa, HCT-116 and U87MG were cultured in the presence of NPs, and cell viability and internalization efficiency in static (standard culture) and dynamic (microfluidic culture) conditions were investigated.Results: Cancer cells cultured with NPs in dynamic conditions were viable and presented higher internalization levels as compared with static 2D cultures.Conclusion: This work demonstrated that the proposed microfluidic-based platform allows real-time monitoring, which upon more studies, namely, the assessment of an anticancer drug release effect could be used for cancer theranostics.