Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape. (June 2017)

• Record Type: Journal Article
• Authors: Alamoudi, Kholod; Martins, Patricia; Croissant, Jonas G; Patil, Sachin; Omar, Haneen; Khashab, Niveen M
• Is Part Of: Nanomedicine. Volume 12:Number 12(2017)
• Page Start: 1421
• Page End: 1433
• Publication Date: 2017-06
• Subjects: bubble generation -- endosomal escape -- gene silencing -- lipoplex -- liposome -- multidrug-resistant cell -- nanomedicine -- siRNA delivery; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2017-0021
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Improving the delivery of siRNA into cancer cells via bubble liposomes. Designing a thermoresponsive pegylated liposome through the introduction of ammonium bicarbonate salt into liposomes so as to control their endosomal escape for gene therapy.Methods: A sub-200 nm nanovector was fully characterized and examined for cellular uptake, cytotoxicity, endosomal escape and gene silencing.Results: The siRNA-liposomes were internalized into cancer cells within 5 min and then released siRNAs in the cytosol prior to lysosomal degradation upon external temperature elevation. This was confirmed by confocal bioimaging and gene silencing reaching up to 90% and further demonstrated by the protein inhibition of both target genes.Conclusion: The thermoresponsiveness of ammonium bicarbonate containing liposomes enabled the rapid endosomal escape of the particles and resulted in an efficient gene silencing.