Three-Dimensional Identification of Microorganisms Using a Digital Holographic Microscope. (31st March 2013)

• Record Type: Journal Article
• Authors: Wu, Ning; Wu, Xiang; Liang, Tiancai
• Other Names: Chen Shengyong Academic Editor.
• Is Part Of: Computational and mathematical methods in medicine. Volume 2013(2013)
• Publication Date: 2013-03-31
• Subjects: Medicine -- Computer simulation -- Periodicals; Medicine -- Mathematical models -- Periodicals; 610.11
• DOI: 10.1155/2013/162105
• Languages: English
• ISSNs: 1748-670X

Abstract

Abstract : This paper reports a method for three-dimensional (3D) analysis of shift-invariant pattern recognition and applies to holographic images digitally reconstructed from holographic microscopes. It is shown that the sequential application of a 2D filter to the plane-by-plane reconstruction of an optical field is exactly equivalent to the application of a more general filter with a 3D impulse response. We show that any 3D filters with arbitrary impulse response can be implemented in this way. This type of processing is applied to the two-class problem of distinguishing different types of bacteria. It is shown that the proposed technique can be easily implemented using a modified microscope to develop a powerful and cost-effective system with great potential for biological screening.